Modification:Article Title: Tissue-derived tissugenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethylxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM (β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal myogenic DMEM/Ham's F-12 medium (or DMEM high glucose), 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/ Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS (fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM (β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal myogenic DMEM/Ham's F-12 medium (or DMEM high glucose), 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate,1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2- mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/ Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I- coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS(fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal DMEM/Ham's F-12 medium (or DMEM high glucose), myogenic 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS (fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal DMEM/Ham's F-12 medium (or DMEM high glucose), myogenic 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10-5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/ Ham's F-12 medium, 10% FCS, 5 × 10-5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10-7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10-7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS (fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham’s F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxy vitamin D3, 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal myogenic DMEM/Ham’s F-12 medium (or DMEM high glucose), 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 uM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham’s F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham’s F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (Fetal bovine serum); FCS (Fetal calf serum Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Suspension:Article Title: Tissue-derived tissugenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethylxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM (β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal myogenic DMEM/Ham's F-12 medium (or DMEM high glucose), 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/ Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS (fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM (β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal myogenic DMEM/Ham's F-12 medium (or DMEM high glucose), 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate,1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2- mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/ Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I- coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS(fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal DMEM/Ham's F-12 medium (or DMEM high glucose), myogenic 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS (fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxyvitamin D3, 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal DMEM/Ham's F-12 medium (or DMEM high glucose), myogenic 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham's F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10-5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/ Ham's F-12 medium, 10% FCS, 5 × 10-5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham's F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10-7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10-7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (fetal bovine serum); FCS (fetal calf serum) Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
Article Title: Tissue-derived tissuegenic implants, and methods of fabricating and using same
Article Snippet: .. The specific culture conditions for differentiation of amniotic stem cells are shown in Table 22: TABLE 22 Culture conditions for differentiation of amniotic stem cells Differentiation Culture Conditions Adipogenic DMEM high glucose (or DMEM/Ham's F-12 medium), 10% FBS, 0.5 mM isobutylmethvlxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin Chondrogenic DMEM high glucose, 1% FBS, 6.25 μg/ml insulin, 10 ng/ml TGF-β1, 50 ng/ml fresh ascorbic acid Osteogenic DMEM high glucose (or DMEM/Ham’s F-12 medium), 10% FBS, 10 μM dexamethasone, 10 nM 1-α, 25-dehydroxy vitamin D3, 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate MesenCult Human Osteogenic Stimulatory Kit (StemCell Technologies) Skeletal myogenic DMEM/Ham’s F-12 medium (or DMEM high glucose), 10% FBS, 5% human serum (or horse serum), 50 μM hydrocortisone (0.1 μM dexamethasone) Cardiomyogenic DMEM, 10% FBS, 55 uM 2-mercaptoethanol, 1 mM sodium pyruvate, 1 mM ascorbic acid 2-phosphate DMEM/Ham’s F-12 medium, 10% FBS, 1 mM ascorbic acid Neurogenic DMEM high glucose, 10% FBS, 30 μM all-trans retinoic acid DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF-4 DMEM/Ham's F-12 medium, 10% FCS, 5 × 10−5 M all-trans retinoic acid, 10 ng/ml FGF4, N-2 supplement (Gibco), B-27 supplement (Gibco) Pancreatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, 10 mM nicotinamide on collagen I-coated plate DMEM (or DMEM/Ham’s F-12 medium) containing N2 supplement (Gibco), 10 mM nicotinamide Hepatic DMEM, 10% FBS, 55 μM 2-mercaptoethanol, 1 mM sodium pyruvate, dexamethansone 10−7 M, 0.1 μM insulin for 3 weeks, addition of 1 mM phenobarbital for the final 3 days, on collagen I-coated plate DMEM, 10% FBS, 20 ng/ml HGF, 10 ng/ml FGF-2, 10 ng/ml oncostatin M, 100 mM dexamethasone, 10 U/ml heparin sodium salt DMEM/Ham's F-12 medium, 10% FCS + 0.1 μM insulin, 1 × 10−7 M dexamethasone Abbreviations: DMEM (Dulbecco's modified Eagle's medium); FBS (Fetal bovine serum); FCS (Fetal calf serum Prior to recellularization, the cryovials containing cryopreserved suspension of amniotic epithelial and/or stromal cells are thawed. ..
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